Thursday, May 19, 2011

A260/A230 ratio

A260/A230 ratio is such an essential ratio to check the purity of nucleic acids besides A260/A280, which is more commonly used and better know, but it is not widely been used.

Wondering, besides protein contamination, do you think that salt (s) or other reagent (s) contamination, which most likely coming from the carry over during the extraction and purification step (s) will actually affect detrimentally to your downstream assay (s)?

If yes, perhaps you should put slightly more attention to A260/230 ratio then......


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