Lots of people will be more concern in data analysis of their qRT-PCR result and will straight away ask me which data analysis method is better than the other so that they can use for their studies. I encountered this question (s) a lot during the installation and training of real time PCR.
However, in most of the case, I found out that they don't even know of or consider the essential of starting material/ sample quality, how to process their samples to get a pure and intact nucleic acids, the quality of their extracted and purified nucleic acids etc etc. These could detrimentally effect the reliability and quality of their data.
And of course, not to mention on the experimental design. Another area which lots of people or researcher (s) overlook its important.
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