22 June, 2011, I went to meet a customer complaining that their real time PCR results were not consistent over the week, where the positive result in one run becames negative in the next run, with the same sample (s).
Before I went, I got some run files from her.
Back in the office, I noticed that there was nothing wrong with the result (s), except that there are some minor differences in her replicates, which could be pipetting error.
To my surprise, after having some discussion with her on how to analyze her result (s), she told me that the way she call a sample as positive or negative based on the exponent from result in the Result column generated by the software; i.e, 2.223e+1 is positive, whereas 2.223e-0.1 is negative.
OMG, she is analyzed her result based on the exponents of the quantitative results generated based on the standard curve she had.
I ended out discussing with her what is the meaning of exponent, and how to interpret the result.
Wow, this is the most creative way of analysis a real time PCR result so far I met.
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